Basic Sciences of Medicine
p-ISSN: 2167-7344 e-ISSN: 2167-7352
2015; 4(2): 21-27
doi:10.5923/j.medicine.20150402.01
Tunmise T. Makinwa , Patrick O. Uadia
Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria
Correspondence to: Tunmise T. Makinwa , Department of Biochemistry, Faculty of Life Sciences, University of Benin, Benin City, Nigeria.
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Bisphenol A (BPA) is one of the endocrine disrupting chemicals. Its estrogenic properties are established; however studies on the effects of BPA on androgen receptors are sparse. We compared the effects of blocking the androgen and estrogen receptors on BPA mechanisms of action in albino rats. Fifty four male albino rats were grouped into nine; group 1 received the vehicle (corn oil), group 2 received flutamide and BPA (100µg/kg/day), group 3 received flutamide and BPA (4mg/kg/day), group 4 received tamoxifen and BPA (100µg/kg/day), group 5 received tamoxifen and BPA (4mg/kg/day), group 6 received BPA (100µg/kg/day) only, group 7 received BPA (4m/kg/day) only, group 8 received flutamide only and group 9 received tamoxifene only. Exposure of rats to a single low (100µg/kg) and high (4mg/kg) dose of BPA induced a rapid decrease in glycemia; which was inhibited by pre-treatment with flutamide but pre-treatment with tamoxifen showed no inhibitory effect. Conversely long-term (12days) injection of BPA (low (100µg/kg/day) and high (4mg/kg) doses) resulted in hyperglycemia. The BPA-induced hyperglycemia was significantly (P<0.05) inhibited by pre-treatment with tamoxifen. Although flutamide injection inhibited the BPA-induced hyperglycemia the effect was not significant (P>0.05). Long-term exposure to high dose (4mg/kg) of BPA resulted in a significant (P<0.05) decrease in serum testosterone compared with the control groups, this effect was not blocked by either flutamide or tamoxifen. This study revealed that both estrogen and androgen receptors are mediators of BPA’s action on glucose metabolism and reproduction.
Keywords: Endocrine disruptor, Bisphenol A, Tamoxifen, Flutamide, Androgen receptor and Estrogen receptor
Cite this paper: Tunmise T. Makinwa , Patrick O. Uadia , Effect of Tamoxifen and Flutamide-Induced Receptor Blockade on Bisphenol a (BPA) Activity in Male Albino Wistar Rats, Basic Sciences of Medicine , Vol. 4 No. 2, 2015, pp. 21-27. doi: 10.5923/j.medicine.20150402.01.
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Figure 2. Showing the area under the curve (AUC) mg/dl/min values in rats injected with the vehicle, 100μg/kg or 4mg/kg body weight BPA, with or without pre-treatment with flutamide or tamoxifen |
Figure 3. Plasma glucose (mg/dl) concentration in rats, injected with the vehicle, 100μg/kg or 4mg/kg body weight BPA for 12 days, with or without pre- treatment with flutamide or tamoxifen |
Figure 4. Serum Testosterone in ng/ml concentration in animals, injected with the vehicle, 100μg/kg or 4mg/kg body weight BPA for 12 days with or without pre-treatment with flutamide or tamoxifen |