World Environment
p-ISSN: 2163-1573 e-ISSN: 2163-1581
2022; 12(1): 1-3
doi:10.5923/j.env.20221201.01
Received: May 4, 2022; Accepted: Jul. 1, 2022; Published: Jul. 15, 2022
Oluwapelumi Shodubi, Jordan Johnson, Jennifer Laifa, Hattie Spencer
Department of Natural Sciences & Environmental Health, Mississippi Valley State University, Itta Bena, USA
Correspondence to: Jennifer Laifa, Department of Natural Sciences & Environmental Health, Mississippi Valley State University, Itta Bena, USA.
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Copyright © 2022 The Author(s). Published by Scientific & Academic Publishing.
This work is licensed under the Creative Commons Attribution International License (CC BY).
http://creativecommons.org/licenses/by/4.0/
Endophytes are bacteria that are found inside plants protecting the plant and enhancing plant growth. They are found in all species of plants, but their relationship is not well understood. The study hypothesized that the endophytes would be found in the roots of pea plants. In the present study, seeds of the pea were purchased from the garden center and grown for six weeks. After six weeks, the plants were harvested, measured, and weighed. The roots were separated from the shoots and roots were cleaned to remove debris. The roots were then sterilized using sterile water and 70% ethanol. The roots were mixed in magnesium sulfate solution using a mortar and pestle. The tryptic soy agar plates were used to grow bacteria. After 24 hours of incubation, the morphology of the colonies was observed. A simple staining procedure was performed using methylene blue. The Gram staining procedure was also conducted. A catalase test was conducted as well. A starch hydrolysis test was also performed. The colonies were also grown on MacConkey agar. The result indicated that the colonies were smooth, and elevated rod-shaped gram-negative bacteria cells. The result indicated that cells were catalase-positive and could not hydrolyze starch. Although the cells grew on MacConkey, they could not ferment lactose. The strain of bacteria was found to be from the genus Pseudomonas. In conclusion, Pseudomonas is one of the bacteria found in the roots of pea plants.
Keywords: Endophytes, Root, Pseudomonas
Cite this paper: Oluwapelumi Shodubi, Jordan Johnson, Jennifer Laifa, Hattie Spencer, An Investigation of Endophytes from Roots of Pea Plant, World Environment, Vol. 12 No. 1, 2022, pp. 1-3. doi: 10.5923/j.env.20221201.01.
![]() | Figure 1. Little marvel pea used in the present study |
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