Clinical Medicine and Diagnostics
p-ISSN: 2163-1433 e-ISSN: 2163-1441
2017; 7(3): 75-79
doi:10.5923/j.cmd.20170703.03
Edward M. Onkendi1, 2, Dorcus A. Abuya1, Ruth M. Mumo1, Leonard King’wara1, Geoffrey K. Kangogo1, Stephen K. Bera1, Nancy N. Bowen1
1Division of National Public Health Reference Laboratories (NPHLS), National Virology Unit & National HIV Reference Laboratory (NHRL), Nairobi, Kenya
2Department of Microbiology, University of Pretoria, Pretoria, South Africa
Correspondence to: Edward M. Onkendi, Division of National Public Health Reference Laboratories (NPHLS), National Virology Unit & National HIV Reference Laboratory (NHRL), Nairobi, Kenya.
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Copyright © 2017 Scientific & Academic Publishing. All Rights Reserved.
This work is licensed under the Creative Commons Attribution International License (CC BY).
http://creativecommons.org/licenses/by/4.0/
Routine HIV-1 viral load monitoring for patients under anti-retroviral therapy is still a challenge in developing countries due to limited access to healthcare facilities and testing services. One of the key challenges hampering access to testing services is the intricate logistics associated with plasma samples required for viral load monitoring. Therefore, there is need to overcome these challenges through new technologies which are affordable and easy to implement. In this validation, EDTA blood samples from 180 HIV-1 patients receiving anti-retroviral therapy were used to prepare dry blood spots and plasma samples for HIV-1 RNA measurements using Roche Cobas Taqman assay. In general, HIV-1 RNA measurements in DBS were lower than those obtained in plasma samples. Detection rates in DBS were 100% in plasma samples with ≥ 3.0 log10 copies/mL. However, plasma samples with ≤ 2.3 log10 copies/mL were not detected in DBS. There was a high correlation (Pearson’s correlation, r = 0.917, P < 0.05) in HIV-1 RNA measurement between dry blood spots and plasma samples. When 12 DBS samples were tested for viral copy numbers after three weeks of storage at room temperature (24°C), there was a high correlation (Pearson’s correlation, r = 0.906, P < 0.05) in HIV-1 RNA measurement between dry blood spots and plasma samples. In addition, there was good concordance between individual plasma and dry blood spot results based on Bland and Altman analysis. Overall, results from this validation suggest that DBS can be used as an alternative sample type for HIV-1 RNA measurements in ART care patients.
Keywords: Dried blood spots, Plasma, Resource-limited, HIV-1 RNA, Regimen failure, Kenya
Cite this paper: Edward M. Onkendi, Dorcus A. Abuya, Ruth M. Mumo, Leonard King’wara, Geoffrey K. Kangogo, Stephen K. Bera, Nancy N. Bowen, Evaluation of Dried Blood Spots as an Alternative Sample Type in HIV-1 RNA Quantification from Patients Receiving Anti-Retroviral Treatment in Kenya, Clinical Medicine and Diagnostics, Vol. 7 No. 3, 2017, pp. 75-79. doi: 10.5923/j.cmd.20170703.03.
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Figure 1. Regression analysis of 23 plasma and DBS samples after collection |
Figure 2. Regression analysis of 12 select plasma and DBS samples three weeks after collection |
Figure 3. Bland and Altman analysis of 23 DBS and plasma samples |