Advances in Life Sciences
p-ISSN: 2163-1387 e-ISSN: 2163-1395
2011; 1(1): 6-10
doi: 10.5923/j.als.20110101.02
A. E. F. Ali 1, M. E. I. Radwan 2
1Department of clinical pathatholgy, Vet. Hospital Benha University
2Department of Infectious Diseases, Vet. Hospital Benha University
Correspondence to: M. E. I. Radwan , Department of Infectious Diseases, Vet. Hospital Benha University.
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Copyright © 2012 Scientific & Academic Publishing. All Rights Reserved.
This investigation is performed on 100 buffaloes native Breed in Kaliobia governorate Egypt aged from 1-3years, these animals suffered from fever (41℃) enlargement lymph node, inappetence, pale mucous membrane, increase lacrimation, emaciation and corneal opacity in progressive stages when these animals were subjected to microscopic examination the degree of parasitaema was recorded as the percentage of infected red blood cells in 1000red blood cell counted .We report the detection of Theileria annulata by PCR in blood samples obtained from buffaloes supposed to be infected. The assay employs primers specific for the gene encoding Theileria annulata 185RNA biochemical changes associated with Theileria annulata in these buffaloes particular emphasis to the oxidative stress the reduce TAC level may reflect adecrease in antioxidant capacity . Blood collected from all animals on EDTA to microscopic examination and PCR to determine type of Theileria.
Keywords: Theileria, PCR, Oxidative Changes
Cite this paper: A. E. F. Ali , M. E. I. Radwan , "Molecular Detection of Theileria Annulata in Egyptian Buffaloes and Biochemical Changes Associated with Particular Oxidative Changes", Advances in Life Sciences, Vol. 1 No. 1, 2011, pp. 6-10. doi: 10.5923/j.als.20110101.02.
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Figure 1. clinical signs of theileriosis in buffalo: A corneal opacity, B Emaciated and enlargement in L.N, C Lacrimation. |
Figure 2. Microscopic examination of giemsa stain blood film showed that: A round, oval piroplasms in erythrocytes, B intralymphocytic shizont of theileria and change in erythrocytes shape. |
Figure 3. Detection of T. annulata in blood samples by PCR, results from 1.5% agarose gel electrophoresis. |
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