1. Introduction

Assistant reproductive technologies (Yort), especially extracorporeal insemination (IUI), today on the day barren couples for the most effective and wide being used from methods one is calculated. The ECU results different factors impact does, of these one in the body homocysteine level. [1] Homocysteine — methionine exchange as a result harvest to be amino acid is metabolic and immunological to processes impact shows. [2] Homocysteine level out of the norm high (hyperhomocysteinemia) or low (hypohomocysteinemia) in the body many systems, including reproductive to health negative impact shows. [3]

In the world Despite the fact that IVF and IVF procedures have been performed for almost 40 years and are constantly being improved, it is still important to find ways to improve the effectiveness of these expensive programs. [4] One of these opportunities is to optimize the preparation of female patients for IVF and IVF treatment, an important part of which is to identify and correct factors that increase the risk of failure. In this regard, it is especially relevant to analyze how various conditions and diseases affect the effectiveness of the method. [5,6]

Current in ECU protocols at the time embryo features with its "quality" It is believed to be related. A row research this showed that the embryo microscopic assessment genetic a relatively normal embryo when choosing information gives and implantation speed increases. [7] Embryo development stages classification modern systems blastomeres number of them one diversity, division speed and decomposition level such as features into account takes. Various diameter blastomeres existence embryo cell of mass uneven, asynchronous to the division take arrival possible.

In recent years, scientific studies have been actively published in the literature on the increase or decrease in homocysteine in women of reproductive age during pregnancy and lactation, and the negative impact of this condition on pregnancy, childbirth and the neonatal period, as well as on the condition of the fetus and newborn [9].

Take visited research this shows that homocysteine level height ovulator cycle to spoil, egg cell quality and number to decrease, embryo implantation level to decrease take [10] Therefore, the ECU cycle from the entrance before homocysteine amount determine, assess and normalization important importance has.

2. Material and Methods

The study will be conducted in Tashkent in 2022–2024. mother " You will be " at the EKU center take went. General 90 people on the account reproductive aged barren woman to check attraction Patients two to the group divided into: 50 people main per group, 40 people control to the group. Home in the group in women homocysteine level identified, high or lower in cases pharmacological and diet methods using normalized. Control in the group in women and this parameter not checked.

In the study following stages and indicators rated: follicular through puncture (TVP) taken oocytes number of them morphological quality (M2), germination efficiency (2PN), embryos development level and quality, pregnancy determiner β -XGCH indicator and clinical pregnancy status.

3. Results

To the study attraction 90 people were killed 50 of the women main group, 40 people and control group organization reached. Home to the group included of patients in all homocysteine level determined, out of the norm aside came out in cases (i.e. hyperhomocysteinemia or hypohomocysteinemia in cases of) treating and corrector measures applied. Control in the group women and the ECU cycle homocysteine level in advance without specifying Research during of patients age, infertility duration, oocytes and embryos quality, pregnancy results, as well as clinical and laboratory parameters was evaluated.

Table 1. Egg cells indicators groups according to comparison

This from the table visible as it stands, general oocytes number main in the group higher Although, at the M2 level mature oocytes control in the group relatively more record However this difference statistic in terms of reliable at the level was not. However main in the group oocytes general number increase, stimulation organism positive answer as evaluation possible.

Table 2. Fertilization and embryo development results

Fertilization efficiency in terms of main group control to the group relatively high to the results achieved. Especially, class A embryos 68.5% of its share enough high good quality embryos that they have reached This means. This is implantation probability increases and ECU cycle to the success positive impact shows.

Table 3. Pregnancy results comparison

Biochemical and clinical pregnancy indicators main in the group noticeable at the level high Homocysteine level normalization in the background embryo implantation and clinical pregnancy level increased. This is diagnostics algorithms homocysteine monitoring input through pregnancy probability increase possible means.

Also, the main in the group embryos development speed, class A affiliation and blastocyst until the stage maturity height record These indicators homocysteine level in moderation was in patients embryogenesis processes better to pass shows.

Treatment strategy algorithm:

1. First stage — to the ECU in preparation all in women homocysteine level is determined.

2. Second stage — if homocysteine is >8.0 or <5.0 μmol /l, pharmacotherapy begins (folate, vitamins B6, B12, SAMe).

3. Third stage — 4–6 weeks from treatment after homocysteine level again is evaluated.

4. Fourth stage — to the standard cited ECG protocol in patients begins.

5. Fifth stage — ECU all stages pregnancy after completion result following will go.

Strategy Importance: This algorithm of patients metabolic profile deep to study is based on and ECU protocol individualized to be carried out This is a barren land. couples for high efficiency, time and financial expenses decrease, as well as psychological relief Homocysteine criterion introduction using high good quality oocytes, optimal embryos and stable to pregnancy achieve opportunity increases.

This results based on To the ECU preparation algorithms homocysteine level input according to clinical recommendations working exit to the goal is appropriate.

4. Discussion

Research. during main in the group homocysteine level normalized after all indicators — oocytes number, fertility level, embryos quality and pregnancy to the surface arrival indicators control to the group than noticeable at the level high It was. This is homocysteine degree before EKU determination and need if correction through final the results improve possible shows.

High homocysteine level endothelial dysfunction, oxidative stress and DNA damage take it comes, this and oocytes and embryos quality Reduces. Low homocysteine level and methylation processes enough to be absent take comes. Both reproductive in any case efficiency decreases.

Research also, homocysteine in moderation to be implantation process success to increase service to do showed that homocysteine correction for folate acid, vitamins B6, B12 and diet therapy effective it has been.

5. Conclusions

In the blood homocysteine level ECU cycle all stages — egg cell maturation, fertilization, embryo quality and to pregnancy noticeable impact shows. This is the case this parameter diagnostics algorithms input necessity Research shows. results based on following treatment algorithm working output.