American Journal of Environmental Engineering
p-ISSN: 2166-4633 e-ISSN: 2166-465X
2017; 7(3): 58-64
doi:10.5923/j.ajee.20170703.02
Michael N. Worfa1, Moses Mensah2, Benjamin Afotey2, Samson P. Salifu1
1Department of Biochemistry and Biotechnology, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
2Department of Chemical Engineering, College of Engineering, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Correspondence to: Benjamin Afotey, Department of Chemical Engineering, College of Engineering, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
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This study investigated the effect of three different types of microbial activity on bioethanol yield from cassava peelings. The cassava peelings were pretreated by milling to 2mm particle size, autoclaved and hydrolyzed using Pleurotus ostreatus k910 (white-rot fungi), Aspergillus niger Menae1 (black molds) and a combination of the two fungi. The hydrolysates obtained were subsequently fermented to ethanol using the Saccharomyces cerevisiae (baker’s yeast). The analysis of lignocellulose fractions, fermentable sugars and bioethanol produced were performed using a van Soest, refractometry and gravimetric methods respectively. The effect of the various fungi used for biological pretreatment/hydrolysis on lignocellulose fractions and fermentable sugars released of each substrate were studied. P. otreatus k910 hydrolysis of the cassava peelings yielded an optimum fermentable sugar concentration of 34.11 g/L compared to 28.64 g/l for the A. niger MENAE1. However, the combination of the two fungi for hydrolysis gave the best results for fermentable sugar produced of 36.51 g/l for cassava peelings. The fermentation results revealed that the maximum ethanol yield for cassava peelings is 19.36% (w/w dry biomass).
Keywords: Second-generation bioethanol, Lignocellulose, Autoclave, Refractometer, Fermentation, Pleurotus ostreatus (white-rot fungi), Aspergillus niger (black molds) and Saccharomyces cerevisiae (baker’s yeast)
Cite this paper: Michael N. Worfa, Moses Mensah, Benjamin Afotey, Samson P. Salifu, Effect of Different Sets of Pleurotus ostreatus and Aspergillus niger Hydrolysis of Cassava Peelings on Bioethanol Yield, American Journal of Environmental Engineering, Vol. 7 No. 3, 2017, pp. 58-64. doi: 10.5923/j.ajee.20170703.02.
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Figure 4. Effect of fermentation of the various cassava peelings hydrolysate with S. cerevisiae on ethanol production (Control = hydrolysate with no Cerevisiae for fermentation process) |
Figure 6. Effect of the type of hydrolysis on percentage ethanol yield of dry biomass |
Figure 7. Comparison of the effect of P. ostreatus k910 and A. niger MENAE1 hydrolysis on cellulose content of cassava peelings (Control=Cassava peelings not inoculated with fungi) |
Figure 8. Comparison of the effect of P. ostreatus k910 and A. niger MENAE1 hydrolysis on hemicellulose content of cassava peelings (Control=Cassava peelings not inoculated with fungi) |
Figure 9. Comparison of the effect of P. ostreatus k910 and A. niger MENAE1 hydrolysis on lignin content of cassava peelings (Control=Cassava peelings not inoculated with fungi) |