The Cytotoxic and Haematological Effect of Coal Dust to Underground Miners Scientific & Academic Publishing: The article detailed information

American Journal of Biochemistry

p-ISSN: 2163-3010    e-ISSN: 2163-3029

2011;  1(1): 4-8

doi: 10.5923/j.ajb.20110101.02

The Cytotoxic and Haematological Effect of Coal Dust to Underground Miners

Meltem Maras Atabay

Department of Secondary Science and Mathematics Education, Biology Education Section, Ereğli Education Faculty,

Correspondence to: Meltem Maras Atabay , Department of Secondary Science and Mathematics Education, Biology Education Section, Ereğli Education Faculty,.
Email:

Copyright © 2012 Scientific & Academic Publishing. All Rights Reserved.

Abstract

This study is aimed to assess the hazardous effect of coal dust and to examine whether there still may be an increased risk of respiratory symptoms among underground miners such as working miners and retired miners in Zonguldak, Turkey. Current miners had to have worked for at least 5 years. Retired miners had worked 10-20 years. Both groups are historically associated with exposure to higher levels of coal dust. Some haematological markers of blood samples taken from coal miners were measured in this study. The variations on haematological markers such as hemoglobin, prolactin, lactat dehydrogenase of these miners were observed. There were higher mean values of hemoglobin levels in both current miners and retired miners. Ca+2 levels as releated with increase of lactat dehydrogenase increase almost 5 times. The differences in prolactin levels were releated with levels of haemoglobin, ferritin and anemia. There may be high risk of respiratory symptoms even in retired coal miners. Measurements of this parameters make an attractive approach for monitoring the effect of coal dust in coal workers. Coal dust together with aging factor effect accelerates to catch respiratory symptoms in the retired coal miners and working miners.

Keywords: Coal miners, Hemoglobin, Lactat Dehydrogenase, Prolactin, Cytotoxicity

Cite this paper: Meltem Maras Atabay , "The Cytotoxic and Haematological Effect of Coal Dust to Underground Miners", American Journal of Biochemistry, Vol. 1 No. 1, 2011, pp. 4-8. doi: 10.5923/j.ajb.20110101.02.

1. Introduction

Coal consists of carbon, hydrogen, oxigen, nitrogen, inorganic minerals, quartz and trace metals[1,2]. The inorganic portion of coal can range from a few percent to > 50% (by weight). Trace metals can include boron, cadmium, copper, arsenic, nickel, iron, antimony, cadmium, cobalt, copper, lead, and zinc, and represent only a very small fraction of the mineral matter. In general, aluminum and iron are the main metals in the coals[3]. Some of these trace elements can be cytotoxic and carcinogenic in experimental models. Common mineral and elemental contaminants are kaolin, mica, pyrite, titanium, calcite, sulfur, sodium, magnesium, and silica. The rank of coal increases from peat to lignite, sub-bituminous to bituminous, and anthracite. As rank increases, the ratio of carbon to other chemicals and mineral contaminants increases[4].
Coal is the most widely used energy source in Turkey. Coal mine beds in Zonguldak province is the biggest coal beds of Turkey[5,6]. Underground coal miners are under high risk due to their jobs. Coal miners have traditionally been exposed to high quantities of dust. Accordingly, dust-related health effects have been verified, such as detrimental influences on lung function and the development of chronic bronchitis. Compared to environmental concent- rations, dust exposure at the workplace can be many times higher, and occupational threshold values are reviewed scientifically on a permanent basis[7]. Inhalation of coal mine dust can lead to the development of several diseases including coal workers' pneumoconiosis (CWP), bronchitis, emphysema, Caplan syndrome, and silicosis[8,9]. Exposure to coal mine dust leads to a significant induction of cytogenetic damage in peripheral lymphocyts of workers engaged in underground coal mining. Cytotoxic quartz dusts induce the secretion of a potent phagocyte activator. The inhalation of coal dust particles is thought to participate in the induction of lung diseases like fibrosis emphysema and cancer[10,11]. The hazardous effect inhalated particles are mediated by the release of inflammatory mediators from activated alveolar macrophages[12]. Inhalated coal dust can deposit in lungs. Silicosis occurs frequently in certain occupations such as mining, sandblasting, surface drilling, stone cutting, construction, pottery making, silica flour mill operations, and other occupations in which silica dust exposures occur.
Some studies showed an increased risc of stomach and lung cancer among coal miners. Coal workers’ pneum- oconiosis (CWP) is the paranchymal lung disease that results from the inhalation and deposition of coal mine dust[13,14].
Trace elements (nickel, lead, cadmium, zinc) in coal dust are cytotoxic[15,16]. Quartz could be a prominent risk factor for lung cancer in coal miners. Mutagenes formed from coal dust induce frame shift mutations. Oxidative DNA-damage was observed to be significantly higher in lymphocyts of coal miners in controls[10].
This study is aimed to asses The prevalence of respiratory symptoms and disease among working miners and retired miners in Zonguldak and to examine whether there still may be an increased risk of respiratory symptoms among under- ground miners after decades of decreased exposure to coal dust.

2. Materials and Methods

2.1. Blood Sampling

Study comprised 75 current miners and 75 retired miners. Retired miners are normally granted a pension at 60 years of age (Table 1). Current miners had to have worked for at least 5 years. Retired miners had worked 10-20 years. Mean working time in the mining areas was 16,75 ± 0,58. They were all smokers. Both group worked as roofbolters and as driller at surface coal mine. Both jobs are historically associated with exposure to higher levels of coal dust. Groups were haematologically investigated. The subjects were asked if coal workers’ pneumoconiosis (CWP) or chronic bronchitis had been diagnosed by physician. Blood samples were taken from cubital venous blood in metal-free vacutainer EDTA tubes.
Table 1. Characteristics of current miner and retired miners
Miners/SizeRetired minersCurrent miners
Sample size (n)7575
Age (years)
Mean ± S.D.67,37 ± 12,9660,54 ± 9,67

2.2. Analysis of Haematological Markers

The following haematological markers were measured: Haemoglobin (HGB), Prolactin, Lactat Dehydrogenase (LDH) as well as other biomarkers. All blood tests were analysed in a central laboratory according to standard haematological methods. Control references values consid- ered for HBG, for prolactin and for LDH levels were 10,7-15,7 µg/dL, 6-30 ng/mL, 240-480 U/L respectively. Descripted statistics (mean ± SD) were analysed by using SPSS software (version 13.0) for hemoglobin, MCHC, LDH, prolactin levels and ages of these miners.

3. Results

In the present study, several coal dusts were investigated to alter haematological parameters of current coal miners and previous miners. LDH, HGB, Prolactin levels were obtained from subjects’ cubital wein. Positive correlation was not found blood HGB levels and age (Figure 1). Differences were considered significant at p<0,05. As shown in Table 2, HGB mean values of HGB levels in current miners were 15,8±0,7 while in retired miners were 13,5±1,1 (P = 0,0013). There were differences between mean values of HGB in current miners compared to retired miners (2,2 ± 1,6).
Figure 1. Haemoglobin (HGB) levels (g/dl) and ages of underground current miners and previous miners
Tablo 2. Mean and S.D. of haematological variables in current miners and retired miners exposed to coal dust
Miners/HeamatologicmarkersRetired minersMean ± S.D.95% CICurrent minersMean ± S.D. 95% CI
Haemoglobin (g/dl)13,5±1,1 12,8 -14,315,8±0,7, 15,3-16,2
MCHC32,2±1,01 31,5-33,030,1±1,4, 29,0-31,2
Prolactin (ng/ml)10,07±2,4 7,22-14,0120,6±3,42, 18,0-23,3
LDH (U/L)385±56,3 338,2-432,4762±147,9 638,8-886,1
Mean values of Haemoglobin concentration (MCHC) levels in the current miners were 30,1±1,4gr/dl (P=0,0002). The retired patients and patient miners aged 82 to 40 were with Resripatory Symptome, Polisitemia and Silicos e.t.c. Mine workers’ systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), and haemoglobin oxygen saturation (HbSatO(2)) were studied. No statistical differe- nces were found for HR, SBP, and DBP (p > 0.05) between working miners and retired miners. HbSatO(2) of working miners (84 +/- 8%) was lower than retired miners (92 +/-5%) (p < 0.05).
The effect of dust exposure on lung function described was lower at retired miners in Zonguldak, Turkey. The nitrogen oxide concentrations were 0.28 ppm (NO) and 0.003 ppm (NO2). Nitrogen oxides (NO x = NO + NO2) showed small but clearly insignificant effects on lung function at retired miners. NO x exposures showed no relevant influence on lung function.
The lowest value of prolactin concentration was 18 ng/ml, the highest value was 23,3 ng/ml in current mine workers (P=0,0001) (Table 2). There were differences between mean values of prolactin in current miners compared to retired miners (10,61±4,4).
Cytotoxicity was also determined by measurement of release of LDH as an indicator of cell membrane damage. A small increase in LDH (4%) was found at the highest concentration in respect to the unexposed cells (2%). Ca+2 levels as releated with increase of LDH increase almost 5 times. Mean value of Ca+2 levels in the patient miners was 9,36±0,26 (P<0,0001) (Figure 2). These result indicate an induction of oxidative DNA damage by exposure to coal dusts of damaged cells and presence of DNA strand-breaks.
Figure 2. Scatter plot showing main relationships found between Calcium (Ca) (mg/dl) and Lactat Dehydrogenase (LDH) (U/L)

4. Discussion

Coal is a fossil fuel mined throughout the world. The generation of coal mine dust during underground coal mining is the most significant source of coal dust exposure. Dust exposure at the workplace can be many times higher. The average cumulative exposure to respirable coalmine dust in Great Britain was 174 gh/m3, which corresponds to an average concentration of more than 3 mg/m3 over 30 years underground[7].
Among the occupations listed by the U.S. Census industry code, coal mining is the highest risk job associated with asthma and COPD death, with a proportionate mortality ratio of 1.98 [95% confidence interval (CI), 1.84–2.12, adjusted for age, sex, and race), compared with the second highest risk job of trucking service of 1.29 (95% CI, 1.22–1.37). There are two basic types of coal mining operations, surface mining and underground mining, producing distinctively different exposure variables and disease entities. Under- ground coal miners are at greater risk of developing CWP than strip or surface miners because of the higher dust levels in the underground environment[4]. Levels of bioavailable iron (BAI) in the coals correlated with the prevalence of CWP. The prevalence and severity of CWP was shown to differ markedly among coal mines despite exposures comparable with respirable dust. CWP prevalence in coal mine regions was shown to be significantly correlated with the levels of BAI from the same region[3].
There is genotoxic risk of occupational exposure to coal mine dust. Reactive oxygen species have been implicated in the pathogenesis of its toxicity. Cellular damage was seen to be associated reactive oxygen. Species with ROS that was occurred by the exposure of coal dust[17]. The high level of HGB means the increase of lung symptom in these patients[11]. Blood levels of HGB and prolactin did not show a normal symmetrical distribution. The differences in prolactin levels were releated with levels of Haemoglobin, ferritin and anemia. The increase of prolactin levels causes irritation of chest wall[18]. The increase of Ca+2 levels in miners was demostrated to be the result of an increased production of LDH. This change in LDH levels is an inducer of plasma membran damages[19,20].
In previous studies, coal is determined to induce cellular damages. The toxicity of elements in coal results from the ability to catalysing occurence of free-radical derived from oxygen interacting with cytoplasmic component and cellular membran. These interactions not only modify potantially functional wholeness, but also occur toxic product modifing various cellular functions[5].
Red blood cell antioxidant parameters were shown to decrease in some stages of coal workers’ pneumoconiosis[21, 22]. Erythrocyte glutathione s-transferase activity and glutathione concentration were decreased in the early stages of pneumoconiosis[23]. Coal dust exposure also affects some serum enzymes. For example, LDH activity increased with this exposure[24-26]. Serum enzymes such as ALT, AST, CK, GGT and LDH levels was showed to be a statisti- cally significant increase (p _ 0.05)[17]. Pinho et al., (2005) [25] were determined high LDH activity in the fluid obtained from the bronchoalveolar lavage following coal instillation.
Castranova and Vallyathan (2000)[4] reported that direct cytotoxicity of silica or coal mine dust exhibits significant response for Lipid peroxidation, Hemolysis, LDH release from alveolar macrophages, Chemiluminescence, Nitric oxide. Cytogenetic analysis at previous studies have showed coal workers have sister chromatid exchanges, chromosomal aberration and micronucleus occurrence. The induction of oxidative DNA damage by long-term exposure to coal dust suggests the oxidative stress as early effect of respirable dust and quartz followed by direct DNA damage due to DNA strand-breaks[10,19,26].

5. Conclusions

This study determined that coal dust exposure caused imbalance at biochemical parameters in blood of coal work- ers. Measurements of these parameters make an attractive approach for monitoring dust induced biologycal phenom- ena in coal workers. Smoking miners had higher risks of physician-dignosed chronic bronchitis and had a tendency to more respiratory symptomes[27,28]. Coal dust together with aging factor effect accelerates to catch respiratory symptoms in the retired coal miners and working miners.

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